【摘要】 本研究从曾见典型禽骨髓性白血病(Myeloid Leukosis,ML)病例的内蒙古某肉种鸡场随机选取的淘汰肉种鸡中,分离出一株J亚群禽白血病病毒(Avian leukosis virus Subgroup J,ALV-J)。利用PCR和间接免疫荧光反应进行鉴定,J亚群禽白血病病毒内蒙株可以被两对ALV-J特异性引物扩增(特异条带约2.2kb和545bp);且在特异性单抗的间接免疫荧光检测中呈现强阳性荧光反应。此外,对山东一例肉种鸡骨髓性白血病病例亦进行了J亚群禽白血病病毒的分离与鉴定。2.2kb PCR扩增物的测序结果表明,两株分离病毒的同源性为96.9％,与己分离的SD9901和YZ9901株ALV-J同源性达94.2％～95.4％。更多还原

【Abstract】 A strain of Avian leukosis virus (ALV-J) subgroup J was isolated from an adult broiler breeder folck, which had a history of myeloid leukosis(ML), in Inner Mongolian. Moreover, another strain of ALV-J was isolated from a ML case in Shandong. The two isolates were positive reaction in polymerase chain reactions with two pairs of primers specific for ALV-J and gave antigenically strong reaction in the indirect fluorescence antibody assay(IFA)with ALV-J specific monoclonal antibody JE9. The sequences of the 2.2kb PCR products of the two strains had 96.9% homology, and had 94.2%～95.4% homology to strain SD9901 and YZ9901 ALV-J.更多还原