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对虾白斑综合症病毒与虾鳃细胞膜特异性结合关系的确立
Verifying the Specific Binding Between WSSV and Shrimp Gill Cell Membrane
【摘要】 <正> 对虾白斑综合症病毒(White spot syndrome virus,WSSV)是养殖对虾的一个主要病原,也是目前发现的基因组最大的动物病毒(基因组约290kDa,双链环状)。WSSV病毒粒子为卵形杆状,外被囊膜,囊膜在尾部延伸成一长尾。它不仅能感染对虾,还能感染其它淡水及海水甲壳类。养殖对虾被感染后,3—10d内累积死亡率可达100%,给对虾养
【Abstract】 Cell membrane extracted from shrimp gill was coated on 96 well plate and bound with DIG labeled White spot syndrome virus (WSSV), then detected with Anti-DIG-Fab-HRP system, meanwhile taking the cell membrane from hepatopancreas, muscle, and E.coli for control group, the result verified the specific binding between WSSV and gill cell membrane. And here the binding assay can be employed as a convenient and rapid way to evaluate the binding nature between WSSV and the shrimp gill cell membrane.
【关键词】 对虾白斑综合症病毒(White spot syndrome virus,WSSV);
细胞膜;
结合分析;
【Key words】 White spot syndrome virus(WSSV); Cell membrane; Binding assay;
【Key words】 White spot syndrome virus(WSSV); Cell membrane; Binding assay;
【基金】 国家重点基础研究发展规划项目课题资助(G1999012002)
- 【文献出处】 中国病毒学 ,Virologica Sinica , 编辑部邮箱 ,2003年03期
- 【分类号】S945
- 【被引频次】14
- 【下载频次】174