【摘要】 将甜瓜属异源三倍体与栽培黄瓜‘北京截头’杂交,经胚胎拯救获得了两个2n:15(14C+1H)的单体异附加系,编号分别为02-17和02-39。02-17植株叶形为戟形,02-39为深陷的掌状形,并且果刺均为白色,不同于异源三倍体的掌状心脏形和黑刺,果形比异源三倍体黄瓜长而更接近普通黄瓜。细胞学研究发现,单体异附加系在终变期和中期I染色体构型主要是由7个二价体和1个单价体构成,出现三价体的频率为4％-5％,中期Ⅱ不均等分裂形成不同的两极,一极染色体为8条,另一极为7条。RAPD分析证明它们分别附加了野生种Cucumis hystrix的染色体。更多还原

【Abstract】 Two monosomic alien addition lines 02-17 and 02-39 (2n=15, 14C+1H) were identified among 52 plants derived from embryo rescue of an Cucumis allotriploid x cucumber. Different from their allotriploid parent, the plants have white spine, and the shape of leaves in 02-17 and 02-39 was hastate and palmate, respectively. In pollen mother cells (PMCs) of 02-17 and 02-39, 7 bivalents and 1 univalent were observed at diakinesis and metaphase Ⅰ. Low frequency (about 4% - 5%) of trivalents has also been observed at metaphase Ⅱ . At metaphase Ⅱ , 8 chromosomes went to one pole while 7 to another. RAPD analysis indicated these two plants were added with one alien chromosome from C. hystrix .更多还原