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农杆菌介导GNA基因转化菜薹
Genetic Transformation of Flowering Stalk with Snowdrop Lectin Gene(GNA)
【摘要】 以菜薹带柄子叶为外植体 ,采用农杆菌介导法将特异启动子PRSs-1与雪花莲凝集素 (GNA)基因构建的嵌合基因转化菜薹 ,获得 2 6株Km抗性植株 ,经过PCR检测和PCR Southern杂交分析证明其为转基因植株
【Abstract】 Cotyledons with petiole of Flowering stalk were transformed with the vector carrying a snowdrop lectin gene( Galanthus nivalid Agglutinin, GNA ).Twenty six transformants were obtained from cotyledons of Flowering Stalk.They were confirmed to be transformants by the PCR and PCR Southern blot analysis.
【关键词】 菜薹;
遗传转化;
雪花莲凝集素基因(GNA);
【Key words】 Flowering stalk; Genetic transformation; Snowdrop lectin gene( GNA );
【Key words】 Flowering stalk; Genetic transformation; Snowdrop lectin gene( GNA );
- 【文献出处】 园艺学报 ,Acta Horticulturae Sinica , 编辑部邮箱 ,2003年04期
- 【分类号】S634.503
- 【被引频次】25
- 【下载频次】123