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草莓外源LEA₃基因的导入

LEA₃ Gene Transferred into Strawberry

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【Author】 Wang Junli 1,2,Ge Huibo 3,Peng Shiqi 3,Zhang Hongmei 1,and Xu Jiuru 2 ( 1College of Life Science,Hebei University,Baoding 071002,China; 2College of Biological Science and Technology,Beijing Forestry University,Beijing 100083,China; 3College of Horticulture,Hebei Agricultural University,Baoding 071001,China)

【机构】河北大学生命科学学院,河北农业大学园艺学院,河北农业大学园艺学院,河北大学生命科学学院,北京林业大学生物科学与技术学院保定071002；北京林业大学生物科学与技术学院,北京100083,保定071001,保定071001,保定071002,北京100083；

【摘要】以草莓 (FragariaananassaDuch .)品种‘Darslect’的花药为试材 ,以MS培养基为基本培养基 ,进行愈伤组织的诱导。通过基因枪法将来源于大麦的胚胎发生晚期丰富蛋白基因LEA3 导入愈伤组织细胞 ,经过除草剂PPT的 4次筛选培养 ,获得了抗性愈伤组织及再生植株。通过地高辛标记的探针进行Southern杂交分析 ,检测到了杂交带 ,表明LEA3 基因整合到草莓染色体基因组中。更多还原

【Abstract】 Callus of anther from strawberry(Fragaria ananassa Duch.Darslect)was induced on MS medium. Late embryogenesis abundant protein gene,LEA 3,from barley(Hordeum vulgare L.)was introduced into strawberry callus by the aid of biolistic-mediated transformation method.After 4 times of PPT selected culture,resistant calli and regenerated plants were obtained.The hybridization bands were determined in different transgenic plants by southern blot,and this showed that LEA 3 gene was integrated into strawberry genome.更多还原