【摘要】 滴注在香蕉 (Musaparadisiacavar.sapientum)叶片表面上的甲基紫精 (MethylViologen ,0 2 5mmol·L-1)液滴 ,经过 5 0 μmol·m-2 ·s-1光照 10h ,可在滴注位点周围形成明显的褐色损伤斑点。通过测定香蕉叶片主要抗氧化酶SOD和CAT活性 ,发现褐斑直径与SOD和CAT活性呈明显的负相关 ,说明甲基紫精褐斑直径与香蕉抗氧化能力之间具有密切的关系。外源活性氧处理引起的叶片膜脂过氧化损伤与甲基紫精褐斑直径呈正相关 ,表明利用甲基紫精滴注法快速衡量香蕉抗氧化能力的可行性。更多还原

【Abstract】 After 10 h illumination under the light of 50 μmol·m -2·s -1,obvious speckles could be formed in the place where the Methyl Viologen was dripped as little blobs on the surface of banana leaves.By determining the activity of two chief antioxidative enzymes,superoxide dismutase (SOD) and catalase (CAT),a significant reverse linear relationship was found between the diameter of Methyl Viologen speckle and the two enzymes activities,showing the close relationship between the speckle diameter and the antioxidative capacity in bananas.Exogenous active oxygen species could induce the damage such as peroxidation of membrane lipid in banana leaves, the damaging degree is closely related with the diameter of Methyl Viologen speckles, this result indicated that the rapid method to evaluate antioxidation capacity in bananas was feasible.更多还原