【摘要】 以中国樱桃半野生种对樱茎尖为外植体进行试管繁殖 ,最佳灭菌方法为二次灭菌 (70 %乙醇30s→ 0 1%新洁尔灭 15min→ 0 1%升汞 3min)结合培养基添加抗生素羧苄西林二钠 5 0mg/L。最佳培养条件 :(1)初代培养基为F14+6 BA 0 5mg/L +IBA 0 2mg/L +GA3 0 5mg/L ;(2 )继代培养基为F14+6 BA0 5mg/L +IAA 1 0mg/L +GA3 0 5mg/L ,碳源采用白砂糖 2 0g/L +山梨糖醇 5g/L。同时研究表明 ,外植体种类、茎尖大小及抗氧化剂维生素C均影响茎尖初代培养成活率。更多还原

【Abstract】 Using the shoot-tips as the explant,the tube propagation of Prunus pseudocerasus was studied.The optimal asepsis and culture condition were established by a statistical method.The explant was asepticized as follows:70% ethanol 30 seconds → 0.1% benzalkonium bromide solution 15 minutes → 0.1% HgCl 2 3 minutes,besides 50 mg/L carbenicillin must be added to the media.The optimal culture condition as follows :(1) the medium for primary propagation contained F14,6-BA 0.5 mg/L,IBA 0.2 mg/L,GA 3 0.5 mg/L;(2) the medium for multiple propagation contained F14,6-BA 0.5 mg/L,IAA 1.0 mg/L,GA 3 0.5 mg/L,2% sugar and 0.5% sorbitol.The results also showed that different explants,the length of shoot-tip and the application of anti-oxidant could affect the surviving rate.更多还原