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番茄ss基因的连锁分析及其种子发芽特性
The Linkage Analysis of ss Gene and Its Effect on Seed Germination in Tomato (Lycopersicon esculentum Mill.)
【摘要】 证明控制番茄种皮表现为海绵状的基因ss符合孟德尔遗传规律 ,并与幼苗绿茎基因ah连锁 ,位于第 9条染色体上 ,使种皮表现为灰白色。来自 95 14 8×红 10 0的F2 代分离群体的种子 ,含有ah基因的无花青素单株个体较有花青素个体普遍表现为前期 (39或 5 1h)发芽率较高 ,而第 10天的平均总发芽率却呈现下降趋势 ;不同单株个体间差异较大 ,总发芽率变化幅度为 5 4 %~ 10 0 % ,表明虽然ah基因可促进番茄种子的前期发芽 ,但还受种子基因型 ,尤其是胚或胚乳基因型的影响。番茄种子种皮褐色色素的积累和ah、aw、aa等基因无关。种皮颜色和茸毛等性状可以作为明显的形态标记 ,直接用于后代个体的选择
【Abstract】 The genetic mechanism of ss gene controlling expression of spongy seedcoat was proved to fit Mendelian fashion in this paper. It was linked with ah , which showed obvious anthocyaninless ( Hoffman ) during seedling stage and located on chromosome 9. Its seedcoat also showed the typical gray white color. The anthocyaninless individuals derived from progeny of the F 2 crossing between 95148 as female parent and Hong 100 as male parent showed higher germinating rate respectively in 39 and 51 hours compared to these individuals with anthocyanin. But the great difference existed among these anthocyaninless individuals which also gave the tendency with low total germinating rate in 10 days. The germinating rate in 10 days in F 2 population varied from 54% to 100%. The results showed that ah gene could improve the prophase seed germinating but it still depended on the seed genotype, especially that of the embryo or endosperm. Further, the accumulation of brown pigment in seedcoat was not related with ah ? aw and aa genes. The described characterization of seedcoat in this paper could be directly used to select the progenies as a visual morphological marker.
- 【文献出处】 园艺学报 ,Acta Horticulturae Sinica , 编辑部邮箱 ,2003年01期
- 【分类号】S641.2
- 【下载频次】97