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长白山赤杨丛枝菌根真菌多样性的半巢式LP-PCR-SSCP检测分析
Semi-nested LP-PCR-SSCP identification of arbuscular endomycorrhizal fungi diversity of Alnus at Changbai Mountain
【摘要】 利用半巢式LP PCR SSCP技术 ,对中国吉林长白山不同海拔生境下 3种赤杨共生丛枝菌根真菌的多样性进行检测分析 .结果表明 ,该地区赤杨属东北赤杨、西伯利亚赤杨及色赤杨共生丛枝菌根真菌在科乃至种的水平上并未随宿主的变化表现出丰富的多样性 ;3个树种在自身属的水平上与共生的球囊霉科 (Glomaceae)至少 1种AMF ,即G .intraradix ,在种的水平上表现出不相关于宿主海拔高度的某种相互选择性 .
【Abstract】 The diversity of arbuscular endomycorrhizal fungi colonized on three species of Alnus at Changbai Mountain was identified based on coupling the sensitivity of semi-nested LP-PCR and the specificity afforded by Single Strand Conformation Polymorphism analysis. It is suggested that altitudes and species of Alnus were not the important factors deciding the distribution of AMF in the grade of family, and there was at least one species of Glomaceae, Glomus intraradix, dominated in Alnus at Changbai Mountain.
【Key words】 Alnus; Arbuscular endomycorrhizal fungi; Semi-nested LP-PCR-SSCP;
- 【文献出处】 应用生态学报 ,Chinese Journal of Applied Ecology , 编辑部邮箱 ,2003年11期
- 【分类号】S718
- 【被引频次】12
- 【下载频次】243