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高温胁迫对柑橘光合速率和光系统Ⅱ活性的影响

Effects of high temperature stress on net photosynthetic rate and photosystem Ⅱ activity in Citrus

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【作者】 郭延平周慧芬曾光辉张良诚

【Author】 GUO Yanping,ZHOU Huifen,ZENG Guanghui,ZHANG Liangcheng ( Department of Horticulture,Zhejiang University,Hangzhou 310029,China)

【机构】 浙江大学园艺系浙江大学园艺系 杭州310029杭州310029杭州310029

【摘要】 用红外CO2 分析仪和叶绿素荧光仪测定了温州蜜柑和脐橙叶片的净光合速率 (Pn)、初始荧光(Fo)、最大光能转换效率 (Fv/Fm)及电子传递速率 (ETR) .结果表明 ,与常温 (2 5℃ )相比 ,高温胁迫 (38~ 4 0℃ )使温州蜜柑和脐橙叶片的Pn、Fv/Fm及ETR下降 ,Fo升高 .胁迫 2 5d后温州蜜柑和脐橙叶片的Pn分别下降 5 5 .6 %和 39.8% ,Fv/Fm下降 2 2 .0 %和 6 .7% ,ETR下降 5 5 .0 %和 4 1.5 % ,Fo分别上升了 113.8%和 14 .9% .柑橘经高温胁迫后 ,在 2 5℃下处理 10d ,叶片的Pn、Fv/Fm、Fo及ETR恢复明显 .这些结果说明柑橘的光合速率下降与PSⅡ反应中心失活有关 .

【Abstract】 Table ADC 225 MK3 CO 2 gas analyzer and PAM 2000 portable fluorescence measurement system were used to measure the net photosynthetic rate ( Pn ), initial fluorescence ( Fo ), maximal photochemical efficiency of PSⅡ ( Fv/Fm ) and electron transport rate ( ETR ) of satsuma mandarin ( Citrus unshiu Marc.) and navel orange ( C. sinensis Osbeck ) leaves. The results showed that exposure of citrus plants to high temperature (38~40 ℃) led to a lowering of Pn , Fv/Fm , and ETR , whereas Fo increased. After exposure to high temperature for 25 days, compared with normal temperature (25 ℃),the Pn of satsuma mandarin and navel orange leaves decreased by 55.6% and 39.8%, Fv/Fm decreased by 22.0% and 6.7% and ETR reduced by 55.0% and 41.5 %, respectively. On the other hand, Fo increased 113.8% and 14.9%,respectively. With subsequent transfer to the 25 ℃ culture room for 10 days, Pn , Fv/Fm , Fo and ETR recovered significantly. These results demonstrated that the reduction of Pn in Citrus was related to the inactivation of PSⅡ reaction center.

【关键词】 柑橘高温胁迫光合作用光系统Ⅱ
【Key words】 CitrusHigh temperature stressPhotosynthesisPSⅡ
【基金】 国家自然科学基金项目 (3 9970 5 2 5 );浙江省自然科学基金资助项目 (3 99470 )
  • 【文献出处】 应用生态学报 ,Chinese Journal of Applied Ecology , 编辑部邮箱 ,2003年06期
  • 【分类号】S666.1
  • 【被引频次】212
  • 【下载频次】971
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