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硫水平对玉米氮、硫代谢特性及根系活性的影响

Effect of sulfate supply level on characteristics of N and S metabolism and on root vigor of corn.

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【作者】 王空军胡昌浩董树亭刘开昌刘存辉

【Author】 WANG Kongjun 1,2 ,HU Changhao 2,DONG Shuting 2,LIU Kaichang 2,LIU Cunhui 2( 1Laboratory of Quantitative Vegetation Ecology, Institute of Botany,Chinese Academy of Sciences, Beijing 100093, China; 2Department of Agronomy, Shandong Agricultural University, Taian 271018, China).

【机构】 中国科学院植物研究所植被数量生态学开放研究实验室山东农业大学农学院山东农业大学农学院 北京100093泰安271018泰安271018

【摘要】 通过Hoagland营养液水培玉米 ,定量研究了供S水平对玉米根系活性和植株S、N代谢特性的影响 .当玉米缺S时 ,玉米根系活性和叶片硝酸还原酶 (NR)活性显著降低 ,植株冠 /根比增大 ;根系与地上部对S、N的吸收均减少 ,S在根系和新生叶中分配相对减少、在衰老叶和茎鞘中分配相对增多 ;N在根系中分配增多 ,新生叶和完全展开叶中分配相对减少 ;植株蛋白质、蛋白氮、蛋白硫、无机硫的含量均显著降低 ,非蛋白氮比率增多 ,无机硫占总S的比率变小 ,全N/全S比增大 ;蛋白质中的N/S比在地上部无显著变化 ,而在根系中显著增大 ;根系对S缺乏的反应更为敏感 .研究表明 ,缺S植株蛋白质含量与NR活性之间呈极显著正相关 ;S的缺乏或过量均影响玉米的N、S代谢 ;以根系作为S素营养诊断器官 ,以N/S比或无机S/全S比作为诊断指标较灵敏 ,正常条件下其数值分别为 10 .7和 0 .3 0 2 .

【Abstract】 The effects of different sulfate supply level on N and S metabolism and on root vigor of corn were quantitatively studied with Hoagland solution under greenhouse conditions.The results showed that in S deficient solution,root vigor and leaf nitrate reductase (NR) activity significantly rdduced,top root ratio (T/R) increased,and S and N accumulation in tops and roots decreased.The partition of S decreased in roots and incompletely expanded leaves,but increased in senescent leaves and sheaths stems ;while the partition of N increased in roots,but decreased in incompletely and fully expanded leaves.The content of protein,protein N,protein S,and sulfate S significantly reduced, percentage of non protein N increased,inorganic S/total S ratio decreased,and total N/total S ratio increased.The protein N/protein S ratio in tops had no observable changes.but increased significantly in roots,showing that root was a sensitive organ to S deficiency.There was a close relatioinship between protein content and NR activity in S deficiency.Deficient or excessive S supply limited N and S metabolism of corn plant.N/S or inorganic S/total S ratio in roots was a sensitive index to indicate the deficiency of S supply,which was 10.7 or 0.302 under normal condition,respectively.

【关键词】 玉米供硫代谢N-S交互作用根系活性
【Key words】 Zea mays L.Sulfate supplyNitrogenMetabolismN S interactionRoots vigor.
【基金】 国家自然科学基金资助项目 (39870 5 2 5 )
  • 【文献出处】 应用生态学报 ,Chinese Journal of Applied Ecology , 编辑部邮箱 ,2003年02期
  • 【分类号】S513
  • 【被引频次】36
  • 【下载频次】399
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