【摘要】 测定了SDS CTAB H2 O Na2 SO4 系统的双水相相图及牛血清蛋白和色氨酸在不同区域双水相中的分配系数 ,研究了相关因素对双水相分相时间的影响规律。结果表明 ,体系的相图中存在 2个双水相区 ,盐的种类、浓度及双水相的两相高度之比是影响双水相分相时间的重要因素。物质的疏水程度越大 ,越易被萃取到富表面活性剂相中 ,水溶性小分子物质不能被萃取。当物质的电性与富表面活性剂相有序组合体的电性相反时 ,分配系数显著增大更多还原

【Abstract】 The cationic anionic surfactant mixtures are able to form an aqueous two phase system(ATPS). The pseudo ternary phase diagram of SDS/CTAB/H 2 O/Na 2 SO 4 system has been examined in the paper. The factors relating the formation time of ATPS and the distribution coefficient of bovine serum albumin(BSA) and tryptophane(Trp) in different ATPS regions have been investigated. It is shown that salt kind and its concentration and the phase volume ratio of system have important influence on the ATPS forming time. It needs at least 30 h for SDS/CTAB/H 2O system to form ATPS. However, the ATPS forming time would reduce to 1 min if there is 0 5 mol/L NaSO 4 in the system. When the ratio of top volume to bottom volume is 1, the ATPS forming time is shortest. The higher the hydrophobicity of the compound, the easier it would be extracted by the surfactant rich phase. Small water soluble compound can not be extracted in ATPS. When the electricity of compound is just opposite to that of the ordered combining structure of surfactant rich phase, the distribution coefficient would be much high.更多还原