【摘要】 目的:建立密骨胶囊中补骨脂素的含量测定方法。方法:采用反相高效液相色谱法(RP-HPLC),色谱条件为:流动相为甲醇-水(43:57),检测波长245nm结果:测得补骨脂素平均加样回收率99.3％,RSD 2.2％。结论:该法操作简便,结果准确,灵敏度高,重复性好,可用于密骨胶囊中补骨脂素的含量测定。更多还原

【Abstract】 Objective:To set up a method for the content determination of psoralen in migu capsules. Methods: RP-HPLC was used for the analysis of samples, with a Hypersil C18 column(5 μm, ID 4.6 mm × 250 mm) ; the mobile phase consisted of methanol and water (43:57), the flow rate was 1 mL·min-1 and the UV detection wave length, 245 run. Results:The average rate of recovery of psoralen in samples was 99.3% ; RSD was 2.2% . Conclusion:The method was shown to be simple and convenient, with accurate results, high sensitivity and good reproducibility. The authors therefore suggest to apply the method for the content determination of psoralen in migu capsules.更多还原