【摘要】 目的　研究吲哚美辛对脂多糖引起的人类风湿性关节炎 (RA)成纤维状滑膜细胞 (FLS)中白介素 6 (IL 6 )表达的影响。方法　用放免分析法检测IL 6蛋白的表达水平 ;RT PCR方法测定IL 6mRNA的表达水平。结果LPS处理对FLS中IL 6表达无显著影响 ;LPS刺激的U937细胞培养液上清液可明显增强FLS中IL 6蛋白分泌及mRNA表达 ;吲哚美辛可显著抑制上述变化 ,且其抑制作用随浓度的增加而增强。结论　吲哚美辛可抑制LPS刺激的U937细胞培养上清液引起的FLS中IL 6的表达更多还原

【Abstract】 Aim To study the effects of indomethacin on interleukin-6 (IL-6) expression stimulated with lipopolysaccharide (LPS)in rheumatoid arthritic patients’ synoviocyte. Methods Fibroblast-like cells (FLS) from rheumatoid arthritic patients’ joint tissue were cultured for 24 h and incubated 24 h with LPS (1 mg·L -1) or the supernatant of U937 cells stimulated by LPS (1 mg·L -1) . After indomethacin or dexamethasone added into the supernatant of U937 cells, FLS was incubated with the super natant for 24 h. The expression of IL-6 protein was detected by radioimmunoassay. The mRNA expression of IL-6 was accessed by RT-PCR. Results LPS did not obviously affect the growth of FLS, and the protein secretion and mRNA expression of IL-6 were not changed in FLS treated with LPS. The IL-6 secretion and IL-6 mRNA expression were significantly increased in FLS cultured with the supernatant from U937 cell treated with LPS. Indomethacin at concentrations of 1×10 -7-1×10 -5 mol·L -1 obviously inhibited the protein secretion and mRNA expression of IL-6 in FLS cultured with the supernatant from U937 cell stimulated with LPS, and the inhibitory effects increased as the concentrations of indomethacin increased. Conclusion Indomethacin can inhibit the increase of IL-6 expression caused by supernatant of U937 cells stimulated with LPS in FLS.更多还原