【摘要】 目的　研究能主动靶向于肝实质细胞的甘草酸表面修饰白蛋白纳米粒的制备工艺。方法　去溶剂化法制备普通纳米粒 ,以高碘酸盐氧化法制备甘草酸 白蛋白纳米粒偶联物。用 2 ,4,6 三硝基苯磺酸显色法与凝胶柱色谱法验证是否偶联成功 ,HPLC法测定修饰纳米粒表面甘草酸密度。结果　修饰纳米粒表面活性氨基数量较对照组减少 1 9 6 % ;偶联于纳米粒表面的甘草酸密度为 9 2 %。纳米粒形态圆整 ,平均粒径为 73nm ,在 2 5℃和 37℃条件下放置 1 0d后 ,性质稳定。结论　甘草酸表面修饰白蛋白纳米粒制备成功 ,为进一步研究其对肝细胞的靶向性奠定了实验基础更多还原

【Abstract】 Aim To study the preparation of bovine serum albumin nanoparticles surface modified with glycyrrhizin(BSA NP GL)targeting to hepatocytes. Methods The bovine serum albumin nanoparticles (BSA NP)were prepared by desolvation process. Glycyrrhizin (GL)was oxidized by sodium periodate to be conjugated to surface reactive amino groups(SRAG)of the BSA NP. The SRAG were quantified by spectrophotometric method using 2, 4, 6 trinitrobenzenesulfonic acid(TNBS). Glycyrrhetinic acid(GA)hydrolyzed from GL,which was on the surface of BSA NP GL was assayed by HPLC after isolation by sephadex G 50. Both methods were used to verify the conjugation achieved. HPLC was used to determine surface density of GL on BSA NP GL. Results The amount of SRAG of the BSA NP GL decreased by 19 6% compared with normal BSA NP. The amount of GL molecule was 9 2% of the total determined SRAG of BSA NP. The mean diameter of the BSA NP GL was 73 nm with round shape. The stability of BSA NP GL was constant when it was stored at 25 ℃ and 37 ℃ during 10 days. Conclusion BSA NP GL was successfully prepared, which is considered to establish an experimental foundation for further research on its ability for targeting to hepatocytes.更多还原