【摘要】 目的　建立同时测定四季青中 3种三萜及其皂苷———长梗冬青苷、地榆皂苷I和救必应酸含量的HPLC分析方法。方法　C1 8柱 ;流动相 :甲醇 水 (5 9∶4 1) ;流速 1 0mL·min- 1 ;检测器 :Sedex 5 5蒸发光散射检测器。　结果线性范围为长梗冬青苷 2 5 6～ 2 5 6 0 μg(r=0 9992 ) ,地榆皂苷I1 6 4～ 16 4 0 μg(r=0 9982 )和救必应酸 3 74～ 37 4 0μg(r=0 9994 )。平均加样回收率为长梗冬青苷 96 3% ,RSD 1 6 % (n =5 ) ;地榆皂苷I 97 3% ,RSD 3 8% (n =5 ) ;救必应酸 97 7% ,RSD 2 1% (n =5 )。 3个化合物的精密度RSD (n =6 )均 <4 % ;重现性RSD (n =6 )均 <5 %。结论　本方法简便、有效、可行 ,可用于四季青三萜及其皂苷的含量测定。更多还原

【Abstract】 Aim To establish an HPLC-ELSD method for the quantification of pedunculoside, ziyuglycoside I and rutundic acid, in the leaves of Ilex purpurea Hassk. Methods By optimizing the chromatographic conditions of HPLC and the parameters of ELSD to study the methodology. Column: Allsphere ODS-2 ( 250 mm× 4.6 mm ID, 5 μm), mobile phase: methanol-water (59∶41), flow rate: 1.0 mL·min -1 , drift tube temperature: 59 ℃, gas flow rate: 2.38 L·min -1 . Results The calibration curves were linear in the range of 2.56-25.60 μg for pedunculoside, 1.64-16.40 μg for ziyuglycoside I and 3.74-37.40 μg for rutundic acid. The average recovery of pedunculoside was 96.3%, RSD 1.59% ( n =5), ziyuglycoside I 97.3%, RSD 3.82% ( n =5), rutundic acid 97.7%, RSD 2.11% ( n =5). All of RSDs of the precision were less than 4% ( n =6), and the reproduciblities less than 5% ( n =6). Conclusion The method is simple, accurate, effective and feasible. It can be used for the determination of the contents of pedunculoside, ziyuglycoside I and rutundic acid in leaf of Ilex purpurea Hassk.更多还原