【摘要】 目的　研究巴豆油致炎小鼠耳部基质金属蛋白酶 9(MMP 9)的表达 ,以及地塞米松、吲哚美辛和白藜芦醇对MMP 9表达的影响。方法　免疫组织化学法测定巴豆油致炎小鼠耳部MMP 9表达 ,明胶酶谱法测定U937细胞MMP 9表达。结果　地塞米松和吲哚美辛以及白藜芦醇对巴豆油引起的小鼠耳肿胀有明显抑制作用 ;对巴豆油引起的小鼠耳部MMP 9表达以及PMA诱导的U937细胞MMP 9表达也有显著抑制作用。结论　巴豆油致炎小鼠耳部MMP 9表达增高 ;地塞米松、吲哚美辛和白藜芦醇的抗炎作用可能与抑制MMP 9表达增高有关。更多还原

【Abstract】 Aim To investigate the expression of matrix metalloproteinase-9 (MMP-9) in mouse ears induced with croton oil and the inhibitory effect of dexamethasone, indomethacin and resveratrol on MMP-9 expression, and further explore the relationship between anti-inflammation and MMP-9 inhibition of these three medicines. Methods Immuno-histochemistry was used to detect the expression of MMP-9 in mouse ears. Expression of MMP-9 in U937 cells was analyzed by gelatin zymography. Results Mouse ear edema induced with croton oil was inhibited significantly by dexamethasone and indomethacin at the dose of 10 mg·kg -1 and resveratrol at 50 mg·kg -1 administered subcutaneously. The inhibitory rate was 76.2% ( P <0.001), 56.7% ( P <0.001) and 36.9% ( P <0.001) respectively. The MMP-9 expression increased in mouse ears induced with croton oil and inhibited by dexamethasone, indomethacin and resveratrol at above doses. Gelatin zymography results showed that MMP-9 expression in U937 cells increased significantly after exposed to PMA at 1×10 -8 mol·L -1 ( P <0.001); MMP-9 expression induced with phorbol myristate acetate(PMA) was inhibited by dexamethasone at 1×10 -9 , 1×10 -7 and 1×10 -5 mol·L -1 , indomethacin at 1×10 -6 and 1×10 -5 mol·L -1 and resveratrol at 1×10 -6 and 1×10 -5 mol·L -1 . Conclusion The inhibition of MMP-9 expression may be one of the anti-inflammatory mechanisms of dexamethasone, indomethacin and resveratrol.更多还原