【摘要】 目的　研究脂多糖 (LPS)及其刺激的U937细胞培养上清液和地塞米松对人类风湿性关节炎 (RA)成纤维状滑膜细胞 (FLS)白介素 6(IL 6)表达的影响。方法　用放免分析法检测IL 6蛋白的表达 ;RT PCR法测定IL 6mRNA的表达。结果　LPS处理对FLS中IL 6表达无显著影响 ;LPS刺激的U937细胞培养上清液可明显增强FLS中IL 6蛋白分泌及mRNA表达 ;地塞米松可显著抑制上述变化 ,且其抑制作用随浓度的增加而增强。结论　LPS刺激的U937细胞上清液使FLS中IL 6表达增加 ;而地塞米松能抑制上述IL 6的变化更多还原

【Abstract】 Aim To study the effects of lipopolysaccharide (LPS), the supernatant of U937 cells stimulated with LPS and dexamethasone on interleukin-6 (IL-6) expression in the synoviocyte from patients with rheumatoid arthritis (RA). Methods Fibroblast-like synoviocytes (FLS) from the joint tissue of patients with rheumatoid arthritis were cultured and incubated for 24 h with LPS (1 mg·L -1 ) or the supernatant of U937 cells stimulated with LPS (1 mg·L -1 ) for 24 h. Dexamethasone was added to the supernatant of U937 cells and FLS was incubated for 24 h. The expression of IL-6 protein was detected by radioimmunoassay. The mRNA expression of IL-6 was accessed by RT-PCR. Results The growth of FLS was not markedly affected by LPS, and the protein secretion and mRNA expression of IL-6 were not markedly changed in FLS treated with LPS. The IL-6 secretion and IL-6 mRNA expression were significantly increased in FLS cultured with the supernatant from U937 cell treated with LPS. Dexamethasone markedly inhibited the protein secretion and mRNA expression of IL-6 in FLS cultured with the supernatant from U937 cell stimulated with LPS. The inhibitory effects were increased as the concentration of dexamethasone increased. Conclusion LPS was not shown to directly affect the expression of IL-6 in FLS, but it indirectly causes the increase of the IL-6 expression in FLS by stimulating U937 cell. Dexamethasone can inhibit this increase of the IL-6 expression.更多还原