【摘要】 目的　建立用毛细管电泳法测定包络物稳定性的方法 ,并以此研究抗菌药鱼腥草素钠与 β 环糊精相互作用的强度 ,测定二者形成的超分子包络物的包络常数。方法　用毛细管电泳法测定鱼腥草素钠的淌度 ,变化缓冲溶液中环糊精浓度 ,通过“电泳峰漂移法”及其数学模型 ,从鱼腥草素钠的电泳淌度差计算获得包络物的包络常数。结果　鱼腥草素钠的淌度随着缓冲溶液中 β 环糊精浓度的增加而增大 ,当淌度到达极限值后 ,不再随 β 环糊精浓度的增加而变化。方法的重现性高。获得包络常数的对数值logKGH=3 81。结论　鱼腥草素钠与 β 环糊精间作用强 ,当1∶1混合时 ,鱼腥草素钠的理论转化率可达 98%。其超分子包络物的包络常数值与 β 环糊精浓度无关。毛细管电泳的电泳峰漂移法可以用于指导药物包络常数的测定 ,进一步可用于包络物在生理条件下的稳定性研究。更多还原

【Abstract】 Aim To establish a method to measure the inclusion constant of supramolecular compound by capillary electrophoresis. In addition, by employing this method to investigate the intensity of interaction of antibacterial sodium houttuyfonate and β-cyclodextrin, and to measure the stability constant of the inclusion compound formed. Methods The mobilities of houttuyfonate were measured in solutions with different concentrations of β-cyclodextrin by capillary electrophoresis. The stability constant of the inclusion compound was calculated by peak-shift model mathematically. Results The mobility of houttuyfonate shifts from its original value with the increased concentration of β-cyclodextrin. When the limit was reached, the mobility of sodium houttuyfonate did not change even by increasing the concentration of β-cyclodextrin. The reproducibility is well, and the logarithm of inclusion constant obtained was 3.81 regardless of β- cyclodextrin concentration added. The interaction of the sodium houttuyfonate and β-cyclodextrin is strong. If they blended at a radio of 1∶1 , the transform rate of sodium houttuyfonate is 98%. The value of stability constant of supramolecular inclusion compound is independent of concentration of β-cyclodextrin. Conclusion This technique of capillary electrophoresis can be used to the measurement of inclusion constant of drug with β-cyclodextrin. Furthermore, it can be applied to research the stability of inclusion compound drugs in physiological condition.更多还原