【摘要】 对深层培养裂褶菌 (SchizophyllumcommuneFr)所获得的裂褶菌素进行了结构研究。经凝胶柱层析 ,聚丙烯酰胺凝胶电泳 ,高效液相色谱分析裂褶菌素为均一组分 ,分子质量 4× 10 4 u。通过完全水解 ,纸层析 ,气相色谱分析单糖组分 ,红外光谱 ,酶解反应 ,高碘酸氧化和固体核磁共振分析结构 ,证明了裂褶菌素是以葡萄糖为单一组分的 β (1 3 ) D葡聚糖 ,其中每 3个葡萄糖分子上连有一个 β (1 6)的分支更多还原

【Abstract】 The structure of schizophyllan from schizophyllum co mmune by submerged cultivation was studied. The purified schizophyllan was proved to be homogeneous with molecular weight 4×10 4 u by sephadex G 200 column chromatography, PAGE and HPLC. Its monomer was determined by hydrolysis, PC, GC and its structure was analyzed by IR, enzymolysis, periodate oxidation, CP/MAS13C NMR. The results showed that schizophyllan was only composed of glucose and it consisted of a backbone chain of 1,3 β D glucopyranose units linked with single 1,6 β D glucopyranoses at about every third glucose molecule in the basic chain.更多还原