【摘要】 从巴西蘑菇子实体中提取的水溶性粗多糖经过脱蛋白、脱色等步骤获得半纯品多糖。依次采用DE AE 纤维素和SepharoseCL 4B柱层析进一步纯化后 ,获得 5种水溶性多糖组分。其中主要组分AB I、AB II b和AB III b得率分别为 3.3%、1.5 2 %、0 .85 %。SephadexG 10 0凝胶层析、旋光度和HPLC鉴定各组分已达到层析纯。更多还原

【Abstract】 Crude polysaccharide was obtained from Agaricus blazei Murill fruiting body by hot water extraction, ethanol precipitation and pronase treatment. Five fractions were identified from the crude polysaccharide by DEAE-cellulose chromatography and Sepharose CL-4B gel filtration. Three main fractions (AB-I, AB-II-b, AB-III-b) were collected finally. The total extraction rates of AB-I, AB-II-b, and AB-III-b were 3.3%, 1.52% and 0.85% respectively. Sephadex G-100 gel filtration,optical rotation examination and HPLC detection showed that the polysaccharides were purified.更多还原