【摘要】 分析重组人甲状旁腺激素 1-3 4的氨基酸组成 ,为该品种的结构鉴定提供依据。水解条件含 1%苯酚的6mol L盐酸 ,10 5℃水解 2 4h ;衍生方法异硫氰酸苯酯 (PITC)柱前衍生法 ;氨基酸测定色谱条件色谱柱为PhenomenexprodigyODS 10 0A。 (4 .6mm× 2 5cm ,5 μm) ;流动相A为 0 .1mol L醋酸钠 (pH6.5 ) -乙腈 (93 :7) ,B为水 -乙腈 (1:4) ;二元梯度洗脱 ;检测波长为 2 5 4nm ;流速为 1ml min ;柱温为 40℃。结果 :实验值与理论值一致 ,偏差小于 10 %。本法准确、可靠、重现性好 ,可用于该产品的质量控制。更多还原

【Abstract】 To analyse the amino acid composition of Recombinant Human Parathyriod Hormone 1-34(PTH1-34) so as to provide the basis for the characterization o f PTH1-34 structure. The Hydrolysis conditions were 1% Phenol and 6 mol /LHCl at 105℃for 24 hours.The derivatization method was phenylisothiocyanate(PITC) preco lum derivatization. The amino acids determination conditions:The column was Phen omenex prodigy ODS 100A?(4.6mm×25cm,5μm).The mobile phase A were 0.1mol/Ls odium acetate solution(pH6.5) and acetonitrile(93:7). The mobile phase B wer e water and acetonitrile(1:4).Gradient elution was used.The detective waveleng th was 254nm. The flow rate was 1ml/min.The column temperature was 40℃. The mea sured value is identical with the theoretical value and error is less than 10%. This method is accurate ,reliable, reproducible and suitable for the quality c ontrol of the drug.更多还原