【摘要】 目的　建立简便、快捷的大量制备质粒的实验方法。方法　以文献报道方法为依据 ,将传统的质粒小量抽提的实验方法与大量抽提的实验方法有机结合 ,以琼脂糖凝胶电泳的方法证明了使用改进后方法的可行性。结果　使用该法可以一次大量地制备质粒DNA ( 690± 2 19) μg ,提取效力为 ( 1 4± 0 4) μg/ml菌液 ,并可直接用于酶切。结论　改进后的实验方法更加简便、快捷、高效 ,为分子生物研究中质粒的大量制备提供了更加实用的实验方法更多还原

【Abstract】 Objective To establish a more convenient technique for large-scale preparation of plasmid DNA from Escherichia coli than before.Methods Based on the literature′s method,the author reasonably combined the conventional small-scale extraction technique with a large-scale one,and then checked the feasibility of the newly improved technique by agarose gel electrophoresis.Results Its production output was (690±219) μg plasmid DNA at one time,extraction rate was (1.4±0.4) μg per milliliter culture medium.The product can be directly digested by restriction enzyme.Conclusion The improved technique is more convenient than before,which provides a practical method for large-scale preparation of plasmid DNA.更多还原