【摘要】 采用L1 2 1 0 细胞株对美丽金丝桃 (HypericumbellumLi)的粗提物及其分步萃取物进行了细胞毒试验 ,结果表明 ,美丽金丝桃的氯仿及乙酸乙酯萃取物具有细胞毒活性 ,其IC50 分别为 5 4和 6 0 μg ml。在细胞毒试验结果指导下 ,同步对美丽金丝桃有效部位进行了化学成分分离。首次从该植物中得到 3个化合物 ,通过理化数据测定及波谱数据分析 ,分别鉴定为 4 ,4 -二甲基 - 7α ,8β -二羟基 - 3,5 -二氧代二环 [4 3 1 ]癸 - 1 (1 0 ) -烯 - 2 -酮 (1 ) ,槲皮素 (quercetin ,2 )及木犀草素 (luteolin ,3)。其中 ,1为一新桥环化合物 ,并通过 2DNMR分析 ,对黄酮类化合物 2及 3的 1 3 CNMR谱中的C5和C9数据指认进行了修正。更多还原

【Abstract】 Cytotoxicity screening (L 1210 ) of extracts of Hypericum bellum was carried out.In the result,CHCl 3 and EtOAc fractions possess cytotoxic activities with IC 50 5 4 and 6 0?μg/ml respectively.A new compound,4,4 dimethyl 7α,8β dihydroxy 3,5 dioxobicyclodec 1(10) en 2 one(1),together with quercetin (2) and luteolin (3),was isolated from the active fractions of H bellum .Their structures were identified by spectral data analysis.All the compounds are reported in this genus for the first time.The 13 C NMR assignments for the C5 and C9 of the two known compounds 2 and 3 were revised by means of 2D NMR experiments.更多还原