【摘要】 天麻抗真菌蛋白 (GastrodiaAntifungalProtein ,GAFP)能强烈抑制腐生真菌菌丝的生长 ,在天麻限制和防止蜜环菌 [Armillariellamellea (Vahl.exFr .)Karst.]侵染球茎的防卫机制中起重要作用。本文报告GAFP抗菌机理研究的部分内容———GAFP对木霉菌丝的作用位点。用荧光试剂异硫氰酸荧光素 (Fluoresceinisothiocyanate ,FITC)标记GAFP ,试验表明 ,标记后的GAFP与未标记的GAFP对木霉菌丝生长均有抑制作用。在荧光显微镜下观察GAFP在木霉菌丝上的作用位点 ,发现被“标记GAFP”作用后的菌丝边缘有荧光 ,并主要集中在木霉菌丝的顶端和菌丝横隔处 ,表明GAFP对木霉的作用位点在菌丝的细胞壁上更多还原

【Abstract】 The Gastrodia antifungal protein (GAFP) can strongly inhibit the mycelia growth of saprophytic fungi and plays an important role in the defence mechanism of plants to restrict and prevent the infection of the fungus Armillariella mellea .This paper reported a part of researches of antifungal mechanism of GAFP--the action site of GAFP on Trichoderma hyphae.GAFP had been labeled by Fluorescein isothiocyanate (FITC). The experiments showed GAFP labeled and GAFP alone both could inhibit the growth of Trichoderma .The action site of GAFP on Trichoderma hyphae had been observed by the fluorescence microscope.Fluorescence had been found on the edges of Trichoderma hyphae affected by the GAFP labeled.Fluorescence from the apex of Trichoderma hyphae and septum were stronger than that from the other place.It indicated that the action site of Gastrodia Antifungal Protein on Trichoderma hyphae was in the cell wall.更多还原