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一株产CoQ₁₀的红假单胞菌的研究

The study of a rhodopseudomonas strain which could produce coenzyme Q₁₀

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【Author】 ZHANG Jia chun 1, YANG Ai ming 2, LIU Gang 2 (1.Institute of Life Science, Yunnan Normal Univercity, Kunming 650092; 2. The Experiment Center of Yunnan Univercity, Kunming 650091; 3. The Test Center of Yunnan Normal Univercity, Kunming 650092)

【机构】云南师范大学生命科学学院；云南大学实验中心；云南师范大学测试中心云南昆明650092；云南昆明650091；云南昆明650092；

【摘要】从滇池分离到一株革兰氏阴性杆菌 ,于光照厌氧及黑暗好氧条件下生长良好 ,活细胞在 870、80 3 nm处有吸收峰 ,具平行片层状的光合内膜系统 ,可归属于红假单胞菌属 (Rhodopseudomonas) ;该菌体中提取分离得到的黄色结晶品 ,经 Craven试验、高效薄层层析、高效液相色谱、紫外光谱、红外光谱、核磁共振及质谱证实 ,为 Co Q1 0更多还原

【Abstract】 A gram negative rod was isolated from the Dianchi Lake, which could grow well either in an anaerobic light or an aerobic dark condition, it had absorption peaks at 870 and 800nm, complex lamellar endomembranes could be seen through electron microscope, it belonged to Rhodopseudomonas. Orange crystals were obtained from the strain and were identified to be coenzyme Q10 by means of Craven test, TLC , UV ,IR, HPLC, 1HNMR, and MS.更多还原