【摘要】 支链淀粉含量是稻米品质的评价标准之一,控制支链淀粉合成的酶是淀粉分支酶.依据GenBank公布的日本水稻淀粉分支酶(Q酶)基因的cDNA序列合成相应引物,应用RT-PCR技术,SOE法成功地克隆到云南合系35号水稻Q酶基因的cDNA全长编码框2464bp.与日本水稻比较,合系35的Q酶基因有16个位点存在差异,并导致10个位点的氨基酸变化.更多还原

【Abstract】 The content of starch-branching is one of the quality estimation standard in rice,the starchbranching synthesize is controlled by the starchbranching enzyme.According to the cDNA sequence of starchbranching enzyme (Q enzyme) gene from Japanese rice that was published in Genbank, we designed and synthesized primers for amplifying this gene. Using the RTPCR and SOE method,we cloned successfully the full reading fram cDNA of the Q Enzyme gene in Yunnan He35 rice,which contain 2?464?bp.Comparing with Q enzyme gene from Japanese rich, there are 16 base variation and 10 amino acid change in Yunnan He35 rice.更多还原