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从腾冲热海免培养获得的5个16 S rDNA克隆分析
Analytical study on five 16 S rDNA clones that come from hot spring in Tengchong with a culture-independent approach
【摘要】 用免培养法(culture-independentapproach)从腾冲大滚锅高温热泉中获得的16SrDNA,经克隆筛选,测定了其中5个克隆的16SrDNA插入片段的近全序列.构建的系统发育树分析结果表明,5个克隆中有2个是Bacillus属,1个是Hydrogenobacter属,1个是Pseudomonas属,还有1个在Thermodesulfobacteriaceae科,介于Geothermbacterium属和Thermodesulfobacteria属之间.
【Abstract】 Five almost completely 16?S rDNA sequences were obtained from Dagunguo of Tengchong Rehai with cultureindependent approach.A phylogenetic tree was constructed,among which two of these sequences are Bacillus,the other three sequences belong to Genera Hydrogenobacter,Pseudomonas and a potential genus that belongs to the family Thermodesulfobacteriaceae,it takes a position between the Genera Geothermobacterium and Thermodesulfobacteria.
【关键词】 热泉;
免培养法;
16SrDNA;
克隆;
【Key words】 hot spring; culture-independent approach; 16 S rDNA; clone;
【Key words】 hot spring; culture-independent approach; 16 S rDNA; clone;
【基金】 科技部基础研究重大项目前期研究专项资助项目(2001CCC00600);国家自然科学基金资助项目(39960003).
- 【文献出处】 云南大学学报(自然科学版) ,Journal of Yunnan University (Natural Sciences) , 编辑部邮箱 ,2003年01期
- 【分类号】Q933
- 【被引频次】5
- 【下载频次】116