【摘要】 目的 :探讨胎盘乙酰基转移酶对妊娠和妊娠高血压综合征妇女乙酰化代谢表型的影响。方法 :采用HPLC法测定尿液内咖啡因代谢产物 5 乙酰氨基 6 甲酰氨基 3 甲基尿嘧啶 (AFMU)和甲黄嘌呤(1X)的相对含量 ,计算二者峰面积比 (PAR) ,绘制概率分布直方图 ,确定健康妇女快、慢乙酰化代谢表型的临界点 (截点 ) ,评价健康妇女 ,妊娠妇女 ,妊娠高血压综合征妇女乙酰化代谢表型的分布特点。结果 :健康妇女快、慢乙酰化代谢表型的截点为 1 .0 5。妊娠妇女和妊高征妇女快乙酰化代谢表型频率分别为 84.8%和 92 .9% ,显著高于健康妇女的 72 % (P<0 .0 5 )。妊高征妇女尿蛋白阳性与阴性者乙酰化代谢表型分布的差异无统计学意义 (P >0 .1 0 ) ,尿蛋白阳性者AFMU 1X值显著低于阴性者 (P <0 .0 1 )。结论 :胎盘乙酰基转移酶影响妊娠妇女乙酰化代谢 ,进而影响其表型分布。常规测定妊娠妇女乙酰化代谢表型 ,可为临床用药提供依据更多还原

【Abstract】 AIM: To study the influence of placenta acetyltransferase (N AT 2) on acetylate phenotypes in pregnant and PIH women. METHODS: T he samples of 20 ml urine were collected 2 h after a cup of 140 mg-caffeine spi ked coffee and the acetylator status were phenotyped by measuring the peak area of two caffeine metabolites, AFMU (5-acetylamino-6-formylamino-3-methylurac il) and 1X (1-methylxanthine) with high performance liquid chromatography (HPLC ). Frequence distribution histograms were drawn to assess and compare slow and f ast acetylate phenotype status among pregnant, PIH, and health women. RESULTS: The subjects were classified as slow acetylators if PAR< 1.05 or as fast if PAR> 1.05 , according to the data obtained from health women. The fast acetylators of pregnant and PIH women were 84.8 % and 92.9 %, re spectively, much higher than that in health women by 72%. There were no signific ant differences between PIH with and without urine protein. The mean AFMU/1X was lower in PIH with unusual urine protein than those without (P< 0.01 ). CONCLUSION: The acetylator status of pregnant women c an be affected by placenta acetyltransferase, and its routine determination may be used to ration drug therapy.utionhisto gramsweredrawntoassessandcompareslowandfastacetylatephenotypestatusamongpregnant,PIH ,andhealthwomen .RESULTS :ThesubjectswereclassifiedasslowacetylatorsifPAR <1 .0 5orasfastifPAR >1 .0 5 ,accordingtothedataobtainedfromhealthwomen .ThefastacetylatorsofpregnantandPIHwomenwere84.8%and92 .9% ,respectively,muchhigherthanthatinhealthwomenby 72 % .Therewerenosignificantdif ferencesbetweenP更多还原