【摘要】 目的　研究慢性乙型肝炎 (慢性乙肝 )患者外周血树突状细胞 (DCs)的状况。方法　外周血单个核细胞 ,在含重组人白细胞介素 4 (rhIL 4 ) ( 5 0 0U/ml)和重组人粒细胞巨噬细胞集落刺激因子 (rhGM CSF) ( 10 0ng/ml)完全培养基中培养诱导出DCs。在显微镜下观察DCs生长状况 ,并采用直接计数法以了解DCs的增殖速度。采用LDH法检测DCs诱导的细胞毒性T细胞 (CTL)杀伤能力。以MTT法检测DCs刺激同种异体混合淋巴细胞反应的能力。结果　慢性乙肝患者外周血单个核细胞中通过诱导培养所产生的DCs的细胞数量明显少于正常人群 ;慢性乙肝患者的DCs的增殖速度与正常人DCs无显著性差异 ,但细胞发育不良。慢性乙肝患者的DCs诱导CTL杀伤作用的能力明显弱于正常人 ;并且在经过体外培养 3、6、9天后 ,慢性乙肝患者的DCs刺激同种异体混合淋巴细胞反应的能力均显著低于同培养时段的正常人DCs。结论　慢性乙肝患者体内的DCs不仅细胞数量明显少于正常人 ,而且发育不良、功能低下。这可能是导致慢性乙肝患者抗HBV特异性免疫功能低下的重要原因之一。更多还原

【Abstract】 Objective To study the status of the dendritic cells (DCs)from peripheral blood of patients with chronic viral hepatitis B(CHB).Methods The peripheral blood mononuclear cells(PBMCs) were cultured in medium with rhIL-4(500u/ml)and rhGM-CSF(100ng/ml).The proliferating speed and growing status of the DCs were surveyed by observing and directly counting under microscope while the DCs were cultured.The DCs ability to stimulate allogenic mixed lymphocyte reaction(MLR) was detected with MTT method and DCs ability to induce CTLs to kill target cells was studied with LDH method.Results The number of the DCs from PBMC of patients with CHB was remarkably lower than that of normal persons.There was no notably difference in the DCs proliferating speed between the CHB group and control grop,but the DCs from CHB patients were of dysplasia.The ability to induce CTLs cytotoxity of patients DCs was markedly inferior to that of the normal persons.On the 3rd,6th and 9th days of cell culturing,the ability to stimulate allogenic MLR of CHB groups DCs was strikingly inferior to that of control groups.Conclusion In the patients with CHB,the DCs are not only less in number,but are immature and inefficient.It can be one of the important reasons for resulting in dysfuction of specific immunity to HBV in the patients with CHB that the DCs status is abnormal.更多还原