【摘要】 目的　了解白细胞介素 2 (IL 2 )诱导外周血单个核细胞 (PBMC)对肿瘤细胞的杀伤效应 ,探讨其作用机制。方法　IL 2 ( 10 5IU/L)体外诱导PBMC 5d ,并与Raji细胞株共同培养 ,采用MTT法测定不同时间诱导后的PBMC对Raji细胞的杀伤效应 ;采用BAS ELISA法检测杀伤活性最大时的IFN γ、TNF α、sFasL的表达 ,采用流式细胞仪检测诱导后PBMC的FasL、穿孔素、颗粒酶B的表达。结果　IL 2 ( 10 5IU/L)体外诱导PBMC 5d时 ,都能明显增强对Raji细胞的杀伤活性 ,而且在培养的第 4天杀伤活性最高 (P <0 0 5 ) ,诱导PBMC 4d后 ,FasL、穿孔素、颗粒酶B的表达与B组相比 ,明显增加 ,差异有显著性 (P <0 0 5 )。结论　IL 2 ( 10 5IU/L)体外诱导PBMC能明显增强对Raji细胞的杀伤活性 ,主要是通过诱导PBMC表达IFN γ、穿孔素、颗粒酶B和FasL的表达而发挥抗肿瘤细胞的作用更多还原

【Abstract】 Objective To investigate PBMC cytotoxicity induced by interleukin 2 (IL 2) to Raji cells and its mechanism. Methods In vitro after inducing PBMC by IL 2 (at the concentration of 100 IU/ml ) and incubating with Raji cells for 5 days, cytotoxicity of induced PBMC to Raji cells line was detected by MTT production of IFN γ, TNF α,soluble Fas L (sFas L) was measured with BAS ELISA and expression of perforin, granzyme B,Fas L was detected with flow cytometer. Results Five days of IL 2 induction enhanced the PBMC cytotoxicity to Raji cells line and the cytotoxicity was the highest on the fourth day( P <0 05). The proliferation and activation of PBMC increased and the expression of perforin, granzyme B,Fas L, IFN γ from PBMC also increased( P <0 05),but the sFas L level did not increase. ( P >0 05). Conclusion IL 2 enhances the cytotoxicity of PBMC to tumor cells and augments the production of IFN γ, perforin, granzyme B, Fas L,but does not increase the expression of sFas L.更多还原