【摘要】 目的　观察腺病毒介导的LacZ基因转移在B1 6细胞的转导效率。方法　腺病毒介导的LacZ基因转导B1 6细胞。结果　当MOI为 50～ 1 0 0 ,即可获得较高的转导效率 ,达 90 %± 1 %。结论　腺病毒介导的基因转移在B1 6细胞能获得较高的转导效率和目的基因的有效表达。提示腺病毒载体作为一种高效的基因转移载体 ,在肿瘤的基因治疗中有着良好的应用前景更多还原

【Abstract】 Objective To observe the transduction efficiency of adenoviral-mediated LacZ gene transfer in the murine melanoma cells B16. Methods The B16 cells was transducted by recombinant adenovirus carrying LacZ gene in vitro. Results Transduction efficiency was as hi gh as 90%±1% at a multiply of infection (MOI) of 50～100.Conclusion Adenoviral-mediated gene transfer has a high transduction efficiency and stab le expression of gene of interest on the murine melanoma cells B16. The results suggest that adenovirus is a high-efficiency gene transfer vector and thus has bright prospect in the gene therapy of tumor.更多还原