【摘要】 目的 :以运动、外源性补充辅酶Q (coenzymeQ ,CoQ)以及运动合并补充CoQ作为干预手段 ,观察线粒体CoQ结合含量、质子跨膜势能与活性氧产生之间的相互关系 ,进一步探讨运动性内源活性氧生成和代谢的线粒体膜分子机制。方法 :雄性SD大鼠 36只 ,随机分为 :(1)安静对照组 (R ,n =6 ) ;(2 )运动中对照组 (Em ,n =6 ) ;(3)力竭对照组 (Ei,n =6 ) ;(4 )安静补药组 (QR ,n=6 ) ;(5 )运动中补药组 (Qm ,n =6 ) ;(6 )力竭补药组 (Qi ,n =6 )。采用三级递增负荷力竭运动模型 ,测定心肌线粒体CoQ9及CoQ10 结合含量、ROS产生速率以及线粒体膜质子泵出与电子传递比值(H+/ 2e)。结果 :(1)Em和Qm组心肌线粒体ROS产生、H+/ 2e和CoQ结合含量分别比R和QR组显著增高 ,并且 ,Qm组三项指标显著高于Em组。 (2 )相关分析表明 ,CoQ结合含量分别与H+/ 2e和ROS产生速率呈线性正相关。结论 :以外源性补充CoQ10 和 /或运动应激作为干预手段时 ,心肌线粒体CoQ含量升高导致建立高质子跨膜势能 ,并进而增加活性氧生成 ,进一步支持“活性氧循环”与Q循环和质子循环并存和共同运转可能是运动性内源活性氧生成及代谢的重要机制更多还原

【Abstract】 Objective In order to further explore the possible mole cular mechanism of exercise -induced endogenous ROS in cludingtheir generation and metabolicpath way ,there lation ships between coenzyme Q (CoQ)binding content ,energytrans for mingrate and ROS generation of hear tmitochondria by regardingexer cise ,CoQ supplement and supplement of CoQ combined with exerci seasef fect factors were observed .Meth ods SD rats were forced torunontread millusing Bedford three -step incremental protocol.The animals were randomly divided intosixgroups :1.Nor malrest control (R) ;2 .Exercisemid way control (Em) ;3.Exhaus tioncontrol (Ei) ;4 .CoQ supplemen trest (QR) ;5 .Supplement of oQ combinedwi the xercisemidway (Qm) ;6 .Supplement of CoQ combinedwi the xhaustion (Qi) .Follo wing parametersin hear tmitochondria wered etermined :1.CoQ9 and CoQ10 binding content;2 .Therate of prot on pumpou tand electr on tran sferof respiratorychain I +III (H+/ 2e)in the present of malate and pyruvate ;3ROS generation rate .Results 1. CoQ 9,CoQ 10 ,H+/ 2e and ROS in Em and Qmweresignifi cant lyhigher thanthose in Rand QRrespectively . These parameters in Qmwer ehigher than those inEmsignificantly .2 .Correlationanalysisshowed CoQ bind ingcontent was positivelyrelated to H+/ 2e and ROS generationre spectively .Conclusion Increasein CoQ binding content of hear tmitochondria during exerciseled to higher protontrans fere lectron -chemistrypotential energy out sidemem brane ,which theng averisetoin crease of ROS generation .Coexistence of“reactiveoxygen cycle”with“Qcycle”and“protoncycle”could bean important mechanism of exercise -induced endogenous ROS generation and metabolism .更多还原