【摘要】 选用18对大熊猫特异的微卫星引物对成都动物园、成都大熊猫繁育研究基地的大熊猫幼子及其母亲和可疑父亲DNA作了PCR扩增。扩增产物在ABI373测序仪上做基因扫描。应用Genotyper672软件处理分型数据,经Cervus2.0软件对亲子关系进行分析。结果表明,在母子关系确实的情况下,18个基因座联合的父亲鉴定排除概率E为0.999921,置信度为95%。而在母子关系不清的条件下,E=0.994109,但置信度低于80%。由于圈养大熊猫的母子关系都是确实的。因此,基因型分析的结果能有效确认出这些大熊猫之间的父子关系。但要准确鉴定出野生大熊猫的亲缘关系,我们还需更多的微卫星标记。更多还原

【Abstract】 We used genotype data across 18 microsatellite loci to establish the paternity of giant panda cubs at the Chengdu Zoo and the Chengdu Research Base of Giant Panda Breeding.The results demonstrate that the combined exclusion probability using these 18 microsatellite loci is 0.999921 while confidence is 95 % when the mother is known;if mother is unknown then the exclusion probability is 0.994109,but the confidence of this is less than 80%.Since the mother-offspring relationship is known in captive populations,the results could resolve unknown paternities in the Chengdu ex situ populations.However,to establish accurately the genetic relationships of wild giant pandas,more microsatellite loci may be required.更多还原