【摘要】 目的　通过检测PLC对家兔血小板释放产物血栓素A2 (TXA2 )、代谢产物前列环素 (PGI2 )以及出血时间的影响 ,以探讨PLC抗血小板聚集的作用。方法　运用放射免疫方法测定以ADP、AA、collagen为诱导剂时 ,PLC对血小板TXB2 、6 Keto PGF1α生成量的影响。常规方法测定PLC对家兔出血时间 (BT)的影响。结果　 6种剂量PLC均能延长出血时间 ,但在给PLC 4h时 ,BT恢复至给药前水平 (P >0 0 5)。以ADP、AA、Collagen为诱导剂时 ,6种剂量的PLC能显著降低TXB2 的生成 (P <0 .0 5) ;以AA为诱导剂时 ,PLC 10 0U·kg- 1及以collagen为诱导剂时PLC 10 0U·kg- 1和PLC 2 0 0U·kg- 1其对TXB2 的生成的抑制作用较ASA组弱 (P <0 0 5) ;以ADP为诱导剂时PLC 10 0～ 40 0U·kg- 1,以AA为诱导剂时PLC 2 0 0～ 60 0U·kg- 1和以collagen为诱导剂时PLC 40 0～ 80 0U·kg- 1其对TXB2 的生成的抑制作用与ASA组相当 (P >0 0 5) ;以ADP为诱导剂时PLC 60 0～ 10 0 0U·kg- 1、以AA为诱导剂时PLC 80 0～ 10 0 0U·kg- 1和以collagen为诱导剂时PLC10 0 0U·kg- 1其对TXB2生成的抑制作用强于ASA组 (P <0 0 5)。 6种剂量的PLC组均明显增加 6 酮 PGF1α的生成量 (P <0 0 5) ,其作用强于ASA组 (P <0 0 5) ,并存在剂量效应关系和时间效?更多还原

【Abstract】 AIM To investigate the antiplatelet effect of PLC by de termining the concentration of TXB 2, 6-keto-PGF 1α and bleeding time( BT). METHODS TXB 2 and 6-keto-PGF 1α were detected by ra dioimmunity kit. Bleeding time were measured by routine methods. RESULTS Six doses of PLC can prolong BT significantly(P<0 05). But 4 h afte r administration, BT recovered to the level before administration. Six doses of PLC inhibited TXB 2 generation when ADP, AA, Collagen were used as revulsant ( P<0 05).The inhibition on TXB 2 generation, of PLC 100 U·kg -1 with AA revulsant, PLC 100 U·kg -1 and PLC 200 U·kg -1 with collagen revu lsant, was less than ASA (P<0 05). The inhibition on TXB 2 generation, of PLC 100～400 U·kg -1 with ADP revulsant, PLC 200～600 U·kg -1 with AA revulsant ,and PLC 400～800 U·kg -1 with collagen revulsant, was compar ative with the ASA (P>0 05). The inhibition on TXB 2 generation, of PLC 60 0～ 1 000 U·kg -1 with ADP revulsant, PLC 800～1000 U·kg -1 with AA revulsant and PLC 1 000 U·kg -1 with Collagen revulsant, is more significant than ASA (P<0 05). 6 doses of PLC can all increased 6-keto-P GF 1α generation more significantly than ASA (P<0 05), and it shows a significant dose-ef fect and time-effect relationship. CONCLUSION PLC prolongs bleed ing time 1 to 2 h after administration until, 4 h after administration. PLC can significantly inhibit TXB 2 generation. but increase 6-keto-PGF 1α gene ration. PLC shows significant antiplatelet effects when administered via duodenu m.更多还原