【摘要】 目的　通过腹外侧视前区 (VLPO)微量注射 5 羟色胺酸 (5 HTP)、非特异性 5 HT受体阻断剂麦角新碱 (MS)和5 HT再摄取抑制剂氟西汀观察 5 HT在VLPO对大鼠睡眠-觉醒周期的影响。方法　采用脑立体定位、核团微量注射和多导睡眠描记技术。结果　VLPO双侧分别微量注射小剂量 5 HTP(0 5μg ,0 1 μl)对大鼠睡眠 -觉醒周期无明显影响 ;而双侧注射大剂量 5 HTP(1 0 μg ,0 1 μl)使大鼠睡眠减少 ,觉醒增加 ;VLPO双侧注射 5 HT再摄取抑制剂氟西汀(6 0 μg ,0 1 μl)可产生与大剂量 5 HTP类似的作用 ;而VLPO双侧微量注射非特异性 5 HT受体阻断剂麦角新碱(MS ,1 0 μg ,0 1 μl)使大鼠睡眠增加 ,觉醒减少。大剂量 5 HTP和MS对觉醒 ,睡眠成分改变具有明显的时间相关性。结论　 5 HT在VLPO参与睡眠 -觉醒周期调节且有促觉醒作用 ,其促觉醒作用可能与突触后物质表达有关更多还原

【Abstract】 AIM To study the role of serot on in (5-HT) in ventrolateral preoptic nucleus(VLPO) on sleep and wakefulness cycl e of rat by microinjection of 5-hydroxytryptaphan (5-HTP , precursor of 5-HT ) , non-special 5-HT receptor antagonist methysergide (MS) and 5-HT retake i nhibitor fluoxetine. METHODS Stereotaxic, microinjection and po lysomnography (PSG) were used in the experiment. RESULTS There was no significant effect by microinjection of 5-HTP(0 5 μg,0 1 μl) into VLPO,but microinjection of 5-HTP(1 μg,0 1 μl)and fluoxetine lead wake i ncreased and sleep decreased; while microinjection of non-selective serotonin receptor antagonist MS lead to the opposited effect. The chang of sleep-wakefu lness cycle caused by 5-HTP or MS were significantly assiociated with time. CONCLUSION 5-HT involved in the regulation of sleep-wake cycle a nd promoted wake in the VLPO and its role of promotion may involve the gene exp ression of post-synaptic neurons.更多还原