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扁蒴藤素对急性实验性炎症的作用及其机制研究

Effect of pristimerin on experimental inflammation in mice and rats

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【作者】 惠斌吴勇杰王鸿田暄

【Author】 HUI Bin, WU Yong-Jie, WANG Hong ,TIAN Xuan(Dept of Pharmacology , Lanzhou Medical College ;Key Laboratory ofTraditional Chinese Drug Preclinical Research of Gansu Province, Lanzhou 730000) National Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000

【机构】 兰州医学院药理学教研室·甘肃省中药新药临床前研究重点实验室兰州大学应用有机化学国家重点实验室兰州大学应用有机化学国家重点实验室 兰州 730000兰州 730000兰州 730000

【摘要】 目的 研究扁蒴藤素对急性实验性炎症的作用及其机制。方法 用巴豆油致小鼠耳肿,角叉菜胶致小鼠足肿,醋酸诱发小鼠毛细血管通透性增高,角叉菜胶致大鼠腹膜炎模型,观察扁蒴藤素对小鼠耳肿、足肿、毛细血管通透性增高以及对大鼠腹膜炎渗出液中的白细胞计数、蛋白质含量、一氧化氮(NO)含量、β-N-乙酰氨基葡萄糖苷酶(NAG)释放、丙二醛(MDA)生成、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性的影响。结果 扁蒴藤素ip 0.156~0.625 mg·kg-1或im 1~4 mg·kg-1明显抑制小鼠耳肿和足肿(P<0.05);im 2~4 mg·kg-1明显抑制小鼠毛细血管通透性增高(P<0.05);im 1~2 mg·kg-1抑制大鼠腹膜炎白细胞渗出、蛋白质渗出及NAG释放,降低NO含量,抑制MDA生成,增强SOD及CAT活性。结论 扁蒴藤素有明显的抗炎作用;抗炎作用可能与其抑制NO生成、清除氧自由基、抗脂质过氧化、稳定溶酶体膜有关。

【Abstract】 AIM To study the anti-inflammatory effects and mechanisms of pristimerin. METHODS Several inflammatory models were established, such as ear edema induced by croton oil, hind paw swelling by carrageenan, elevation of capillary permeability by acetic acid in mice and a-cute peritonitis induced by carrageenan in rats. Protein content was measured by Coomassie brilliant blue method, nitric oxide (NO) content by Griess reaction assay, N-acetyl-β-D-glucosamini-dase (NAG) activity by colorimetry, superoxide dismutase (SOD) activity by hydroxylamine method, catalase (CAT) activity by ultraviolet spectro-photometry, and malondialdehyde (MDA) content by fluorescence method in peritoneal exudate in rats. RESULTS Pristimerin ip 0. 156 - 0. 625 mg μ kg-1 or im 1-4 mg - kg-1 inhibited ear edema, hind paw swelling, and elevation of capillary permeability in mice. In the rat peritonitis induced by carrageenan, pristimerin im 1 - 2 mg ·kg-1 reduced neutrophil counts, lessened protein and NO content, inhibited the production of MDA and decreased NAG activity, while augmented the SOD and CAT activity in exudate. CONCLUSION Pristimerin has a significant anti-inflammatory effect which may be related to the inhibition of NO production, scavenging oxygen free radicals, anti-lipoperoxidation and stabilizing lysosome membrane.

【关键词】 扁蒴藤素炎症自由基
【Key words】 pristimerininflammationfree radi-cals
  • 【文献出处】 中国药理学通报 ,Chinese Pharmacological Bulletin , 编辑部邮箱 ,2003年06期
  • 【分类号】R285
  • 【被引频次】33
  • 【下载频次】239
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