【摘要】 目的 应用竞争性酶联免疫定量检测磷酯酶C（PLC）对人血小板膜糖蛋白Ib（GPIb）量的变化,探讨用药后抑制血小板粘附作用的机制。方法 用竞争性酶联免疫药盒测定并绘制标准曲线图;取健康人血制备洗涤血小板,将其分为4组:①生理盐水组;②PLC 0.5 U组;③PLC 1.0U组;④PLC 2.0 U组,分别替代药盒中标准IgG（IgG-ST）进行实验,酶标仪上测OD₄₉₂值,共实验6人次,依据OD₄₉₂值在标准曲线图和Eocel软件求出GPIb的量及 用t值检测它们之间的相关性。结果 用生理盐水、PLC0.5 U、1.0 U和 2.0 U组的OD₄₉₂值和GPIb数量（ng）分别为0.768±0.078、1.103±0.118、1.367±0.102、2.055±0.453和134.669±13.144、101.838±9.879、82.632±5.354、73.183±14.740。PLC 1.0 U和2.0 U组与生理盐水组比差异有显著性（P<0.01）,而其它各组之间比差异无显著性（P>0.05）。结论 PLC减少GPIb的量,从而抑制血小板粘附性,这也可能是PLC抗血小板聚集作用的重要原因之一。更多还原

【Abstract】 AIM To explore mechanism of PLC inhibition on platelet adhesion by quantitive determination of PLC influence on human platelet membrane GPIb quantity (ng) with competitive enzyme-linked immunosorbent assay (CELISA). METHODS Standard curve are drawn with CELISA kit. Washing platelet are made from healthy human blood and divided into four parts: (1)physiological saline part;(2)PLC 0. 5 U part; (3)PLC 1. 0 U part; (4)PLC 2. 0 U part to replace standard IgGCIgG-5T). Then OD492 are tested with TECAN. The experiment was made for 6 times. GPIb quantity(ng) was calculated according to OD492 by standard curve and Eocel. Their correlation are tested with t value. RESULTS OD492 of physiological saline, PLC 0. 5 U, 1.0 U, 2. 0 U were 0.768 ± 0.078, 1.103±0.018, 1.367 ± 0.102, 2.055 ± 0.453 respectively, and their corresponding GPIb quantities (ng) are 134.669 ± 13.144, 101.838 ± 9.879, 82.632 ± 5.354, 73. 183?±14. 74 respectively. The differences of GPIb quantities are significant a-mong PLC 1. 0 U , PLC 2. 0 U and physiological saline ( P < 0. 01), but not notable among other parts(P>0. 05). CONCLUSION PLC can reduce platelet membrane GPIb quantity apparently, so it can inhibit platelet adhesion. It may be one of the important reasons of PLC anti-aggregation on platelet.更多还原