【摘要】 目的　测定血清中氢化可的松和可的松的比值以反映人体肝脏 1 1 β -羟类固醇脱氢酶的活性 ,为其研究提供一个有效的分析手段。方法　HPLC -UV法 ,梯度洗脱 ,内标定量。结果　血清中氢化可的松在 50～ 2 0 0μg·L-1 、可的松在 1 0～ 50 μg·L-1 范围内其线性回归方程分别为Y =- 0 .42 5 +0 .0 1 0X、Y =- 0 .1 63 +0 .0 50X ,相对回收率分别为 (1 0 2 .5± 3 .6) %、(97.6± 6 .0 ) %。 82名健康志愿者血中氢化可的松和可的松的浓度分别为 (1 32 .2± 44 .5) μg·L-1 和 (1 9.7± 5 .0 ) μg·L-1 ,其比值为 6 .89± 2 .2 6。结论　本法操作简便、灵敏可靠 ,符合人体血样糖皮质激素测定的要求更多还原

【Abstract】 Objective To investigate the activity of 11β-hydroxysteroid dehydrogenase, an effective analytic method for measuring the ratio of cortisol and cortisone was developed. Methods Gradient HPLC was used. Results The linear regression equations for cortisol and cortisone were Y =-0.425+0.010 X and Y =-0.163+0.050 X ; the percentages of their average recovery were(102.5±3.6)% and(97.6±6.0)%, respectively. The serum concentrations of cortisol and cortisone in 82 healthy volunteers were(132.2±44.5) μg·L -1 and (19.7±5.0) μg·L -1 , and the ratio of cortisol and cortisone was 6.89±2.26. Conclusion This method can satisfy the requirements for measuring human serum glucocorticoid, being simple, sensitive and credible.更多还原