【摘要】 目的　探讨药理浓度的三氧化二砷 (As2 O3)对雄性大鼠生殖功能的影响。方法　应用不同剂量的As2 O3(2、 4和 8mg·kg- 1 ·d- 1 )给大鼠腹腔内注射 ,2周后对大鼠进行睾丸组织病理学检查 ,同时检测大鼠血清砷的含量 ,睾丸精子头计数 ,并计算每日精子生成量。结果　随着给大鼠注射As2 O3的量增加 ,血清砷浓度也明显增加 ,同时大鼠睾丸精子头数和每日精子生成量也逐渐减少 ,在大剂量组出现显著统计学差异 (P<0 0 0 1) ;血清砷浓度与睾丸精子头数成负相关 (P小于0 0 1) ;小剂量组形态与正常对照组相似 ,中剂量组曲细精管结构变化虽不明显 ,但精子形成有所减少。而大剂量组部分曲细精管出现破裂、渗出 ,基膜溶解 ,精子生成明显减少 ,间质出现水肿渗出 ,支持细胞和各级生精细胞也有所减少。结论　As2 O3可通过损伤大鼠睾丸的组织结构、抑制睾丸精子生成而产生生殖毒性。更多还原

【Abstract】 Objective: To explore the effects of arsenic (As 2O 3) of pharmacological concentration on histological structure changes of testis and sperm production in rats. Methods: Different doses of As 2O 3 (2, 4和8 mg·kg -1 ·d -1 ) were administrated i.p. to the adult male rats. After 2 weeks, the morphological changes of testis were observed, serum content of arsenic was determined; the numbers of testicular sperm head were counted and daily sperm production was calculated. Results: The number of testicular sperm head and daily sperm production in large?doses group decreased more significantly than that of control (P=0 000). There were negative correlation between the content of serum arsenic and the sperm head counts(r=-0 515, P<0 01). The morphology of testis in mid?doses group had not significantly changed, but the form of sperm decreased. In large?doses group, there were break and effusion in part seminiferous tubule. The dissolution of basement membrane, the form of sperm decrease, the edema and effusion of testicular mesenchyme, the decrease of Sertoli cells and every stage spermatogenic cells were observed by light microscope. Conclusion: By means of damaging histological structure changes of testis and restraint of sperm production in male rats, As 2O 3 may give rise to male reproduction toxicity.更多还原