【摘要】 目的:探讨慢性HBV感染患者HBVDNA前C区变异的临床意义。方法:采用ELISA测定99例HBV感染患者血清肝炎病毒标志物,并采用RT-PCR检测HBVDNA前C区变异。结果:99例HBV感染患者中,乙型肝炎病毒携带26例,慢性乙型病毒性肝炎61例,慢性重型病毒性肝炎(乙型)11例和急性乙型病毒性肝炎1例。乙型肝炎病毒携带,慢性乙型病毒性肝炎和慢性重型病毒性肝炎中,变异株组HBVDNA前C区变异发生率与混合株组、野生株组相比,差异均无显著性(P>0.05)。与混合株和野生株两组相比,HBVDNA变异株组肝功能变化差异均无显著性(P>0.05)。结论:HBVDNA前C区变异可发生于HBV感染的不同临床状态。HBVDNA前C区变异可能与肝炎程度和病情进展无明显关系。更多还原

【Abstract】 Objectives:To explore the clinical significance of HBV DNA pre-core mutation in chronic hepatitis B virus infection. Methods:ELISA was used to detect serological markers of HBV, and RT-PCR was performed to determine HBV DNA precore mutation in 99 patients with chronic hepatitis B virus infection. Results:Of the 99 patients, chronic HBV carrier was found in 26 cases, acute hepatitis B in 1, chronic hepatitis B in 61, and chronic severe hepatitis B in 11. No significant difference in occurrence rate of blend, wild-type, and mutant was found in chronic HBV carrier, chronic hepatitis B, and chronic severe hepatitis B, respectively (all P>0.05). Compared with blend-group and wild-type group, there were no significant difference in serum levels of SB,ALT,AST,TBA,and ALB in HBV DNA mutant group, respectively ( all P>0.05).Conclusion:HBV DNA pre-core mutation occurs in various status of chronic HBV infection, including chronic HBV carrier, acute hepatitis B, chronic hepatitis B, and chronic severe hepatitis B. It may not be associated with the pathological change and prognosis of HBV infection.更多还原