【摘要】 目的　 8 氯腺苷对HL 6 0细胞增殖抑制作用及其机制的探讨。方法　采用MTT和SRB方法检测了 8 氯腺苷对不同种类人肿瘤细胞增殖的作用 ;通过扫描电镜和透射电镜观察了HL 6 0细胞表面和核的变化 ;利用NBT还原和酸性磷酸酶分析诱导HL 6 0细胞分化 ;用流式细胞仪分析了 8 氯腺苷对HL 6 0细胞周期的影响。结果　 8 氯腺苷对七种人肿瘤细胞的增殖有抑制作用 ,其IC50 值为KB ( 0 0 5 μmol·L- 1 ) <HL 6 0 ( 0 2 5 μmol·L- 1 ) <Bel 74 0 2 ( 0 5 6 μmol·L- 1 ) <MCF ( 0 6 5 μmol·L- 1 ) <HCT ( 0 79μmol·L- 1 ) <Hela ( 0 89μmol·L- 1 ) <BGC 82 3( 1 4 9μmol·L- 1 ) <PG ( 2 5 0 μmol·L- 1 )。电镜结果显示 ,8 氯腺苷 ( 0 5μmol·L- 1 )与HL 6 0细胞作用 72h ,HL 6 0细胞表面微绒毛变短 ,细胞核型发生了改变 ,如马蹄、肾和分叶型。同时 8 氯腺苷可促进HL 6 0细胞对NBT的还原能力及增加细胞中酸性磷酸酶的活性 ,其作用具有时间和浓度依赖性 ,说明 8 氯腺苷可诱导HL 6 0细胞的分化。细胞周期分析证实 ,8 氯腺苷使HL 6 0细胞G1 期增多 ,S期细胞减少。结论　 8 氯腺苷可以诱导HL 6 0细胞的分化 ,并阻止HL 6 0细胞停止在G1 期 ,从而导致HL 6 0细胞增殖抑制。更多还原

【Abstract】 Aim To study the effect of 8 chloroadenosine (8 CA)on undifferentiatied HL 60 cell line. Methods The IC 50 of cancer cell proliferation was determined using a microculture plate reader at 570 nm (MTT) and 540 nm (SRB). Morphology of HL 60 cells was observed under a scanning electron microscope and a transmission electron microscope. The differentiation of HL 60 cells was examined by nitro blue tetrazolium reduction (NBT) and acid phosphatase assay. The cycle of HL 60 cells was analyzed by flow cytometry. Results 8 CA inhibited proliferation of eight human cancer cell lines. The IC 50 ranked in the following order: KB (0 05 μmol·L -1 ) < HL 60 (0 25 μmol·L -1 ) < Bel 7402 (0 56 μmol·L -1 ) < MCF 7 (0 65 μmol·L -1 ) < HCT (0 79 μmol·L -1 ) < HeLa (0 89 μmol·L -1 ) < BGC 823 (1 149 μmol·L -1 ) < PG (2 50 μmol·L -1 ). The scanning and transmission electron microscopy showed that the microvilli of HL 60 cell surface shortened, and the shape of HL 60 cells nuclei changed to kidney shaped, horse shoe shaped and bilob ated after treatment with 8 CA. Meanwhile, 8 CA promoted NBT reduction and increased activity of acid phosphatase in HL 60 cells in a time and concentration dependent manner. Flow cytometry analysis indicated that 8 CA induced an appreciable increase of the cell population in G 1 phase with a marked reduction in S phase. Conclusion 8 CA can induce differentiation of HL 60 cells and block the cells at G 1 phase, thus inhibiting proliferation of HL 60 cells.更多还原