【摘要】 探讨外源性碱性成纤维细胞生长因子(hFGF),对缺氧缺血性脑损伤(HIBD)新生大鼠脑c-fos基因蛋白产物(c-Fos)表达影响,研究原癌基因c-fos表达与HIBD的关系,采用免疫组织化学染色及定量图像分析方法,观察c-Fos表达强度、染色灰度及面积的变化。结果显示:正常生后7-14 d新生大鼠脑有c-Fos表达,以生后10 d最为明显;缺氧缺血20 min后即刻c-Fos表达增强,并于缺氧缺血后4 h达高峰,持续至72 h;脑内不同部位c-Fos表达时间、强度、灰度及面积不同。连续腹腔注射外源性bFGF 3 d后鼠脑c-Fos表达增强。结论:外源性bFGF可增强HIBD新生大鼠脑c-Fos的表达,bFGF及c-fos基因可能参与HIBD修复的病理生理过程。更多还原

【Abstract】 To investigate the effects of exogenous basic fibroblast growth factor (bFGF) on c - fos gene protein expression (c - Fos) during hypoxic - ischeniic brain damage (HIBD) in neonatal rat brain and the relationship between c - Fos expression and HIBD. eicemunatin on c - Fos expression was done by immuno-histochemical staining and image quantitative analysis. Results showed that c - Fos was expressed in brain of normal neonate rats on postnatal day (PND) 7 to 14 with maximal expression on PND10. c - Fos expression were increased in different parts of brain immediately after hypoxia - ischemia (HI) for 20 min and peaked at 4 hours, and thereafter, its density gradually decreased, which lasted until 72 hr after HI insult, c - Fos expression was obvious in Ⅱ-Ⅴ layers of cortex, CA1 and DG of hippocampus, respectively . The time course, density of the expression, gray degree and area of c - Fos - positive cells varied with different cerebral areas, and that of thalamus was slightly increased. Expression of c - Fos was up -regulated by intraperitoneal bFGF administration. Conclusions: Exogenous bFGF up - regulates the expression of c - fos gene protein in neonate rat brain in HIBD. bFGF and c - fos gene may take part in pathophysiological process of HIBD.更多还原