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柑桔种子贮藏蛋白基因部分序列的克隆
Cloning of the Seed Storage Protein Gene in Citrus Sinensis cv.Jincheng
【摘要】 应用染色体步移技术从柑桔优良品种锦橙(Citrussinensiscv.Jincheng)的总DNA中获得一条长度为2054bp的片段.序列分析结果表明,该片段含有柑桔种子贮藏蛋白基因(citrin)的5 端部分序列,并且它与同一物种的伏令夏橙(C.sinensiscv.Valencia)的citrin是高度同源的.从而证明,已克隆并获得了锦橙种子贮藏蛋白基因的部分序列.
【Abstract】 5’-upstream region of the gene encoding seed storage protein in C. sinensis cv.Jincheng was isolated by using Genomic Walking and then sequenced. The amplification fragment is about 2 kb in length, its sequence was highly homologous to the seed storage protein gene of Citrus sinensis cv.Valencia. The result demonstrated that this sequence was a part of the seed storage protein gene of citrus.
【关键词】 种子贮藏蛋白基因;
基因克隆;
序列分析;
锦橙;
【Key words】 the seed storage protein gene; gene cloning; sequence analyse; Citrus sinensis cv. Jincheng;
【Key words】 the seed storage protein gene; gene cloning; sequence analyse; Citrus sinensis cv. Jincheng;
【基金】 国家自然科学基金资助项目(30270929);重庆市科委资助项目.
- 【文献出处】 西南师范大学学报(自然科学版) ,Journal of Southwest China Normal University(Natural Science) , 编辑部邮箱 ,2003年06期
- 【分类号】Q78
- 【被引频次】8
- 【下载频次】98