【摘要】 毛葡萄组织培养,通常存在玻璃化和成本高、成活率低等问题,不利于产业化生产,从技术路线入手,探索毛葡萄产业化育苗新工艺,经多年研究及生产应用表明:在1/2MS+BA0 01～0 05+IAA1 0～2 0培养基上,培养光照强度1500～2000lux,温度27～30℃,采用组培微型扦插方式,可以实现毛葡萄组培育苗产业化。以塘泥:椰糠或河沙=2∶1为基质,假植成活率可达90%以上。更多还原

【Abstract】 The problems of traditional tissue culture of Vitis quinquangularis Rehd,including vitrification,higher cost and lower survival rate,disadvantaged commercial application of the culture,The new propagating technology of the plant was resulted from the studies and production practices with different technology routes for years.With the medium of 1/2 MS+BA 001-005+IAA 10-20,1500-2000 lux of illuminatiom and 27-30?℃ of temperature,the commercial application of the culture could be benefited by the way of microcuttage.The survival rate of the clone transfer could attain more than 90?%in the media of pond silt:coconut cake or sand=2∶1.更多还原