【摘要】 根据家蚕(Bombyxmori)滞育激素-性信息素合成激活肽(DH-PBAN)基因的DNA序列设计引物,以中国野桑蚕(BombyxmandarinaChina)基因组DNA为模板进行PCR扩增。试验首先确立了比较稳定的从基因组扩增目的片段的扩增体系及程序,并用该扩增体系及程序克隆到中国野桑蚕DH,PBAN及DH-PBAN基因的第5内含子序列。试验还发现该基因第3内含子在家蚕和野桑蚕之间表现出多态性。更多还原

【Abstract】 Specific primers are designed according to the DNA sequences of the gene encoding precursor protein of diapause hormone-pheromone biosynthesis activating neuropeptide (DH-PBAN) of silkworm (Bombyx mori) to amplifiy the genome DNA of Chinese wild silkworm (B. mandarina) and various strains of silkworm. A stable system fitting for amplifying the aimed gene from genomic DNA is determined. Using the system and program, the fifth instron of the PCR products of DH, PBAN and DH-PBAN is obtained and the third instron of the polymorphism of DH-PBAN gene is discovered.更多还原