【摘要】 目的 :研究高原脂肪栓塞综合征 (FES)模型制作及其急性肺损伤预警指标。方法 :1 4只犬在模拟 40 0 0m高原随机分为FES组与对照组。FES组以 1 3ml/kg同种脂肪液静脉注射。结果 :伤后犬立即呼吸窘迫 ,PaO2 下降 ,双下肺可闻及湿性罗音 ,病理检查见肺内广泛粒细胞浸润、脂肪栓塞、肺泡水种。 (PaO2 /F1 0 2 )× (吸入气体压 /平原空气压 ) <30 0。结论 :以 1 3ml/kg同种脂肪液静脉注射可建立较好的犬高原FES模型。 (PaO2 /F1 0 2 )× (吸入气体压 /平原空气压 ) <30 0 ,对高原FES诊断有一定帮助。伤后早期呼吸窘迫 ,外周血PMN和血小板计数及FN含量下降 ,PaO2 下降 ,GPT、共轭二烯、唾液酸的一过性升高 ,对高原FES的发生有一定预测意义更多还原

【Abstract】 Objective:To establish an animal model of fat embolism syndrome(FES)at high altitude and investigate the premonitory index of acute lung injury induced by FES Methods:Fourteen dogs were randomly divided into FES group and control group at simulated high altitude WBC and platelet in peripheral blood and polymorphonuclear nertrophil leukocyte(PMN)and alveolar macrophage(AM)in bronchoalveolar lavage fluid(BALF)were counted by regular means Glutamate -pyruvate transaminase(GPT)value was determined by MCA The level of conjugated dienes(CD)and sialic acid(SA)were detected according to the reference Fibronectin(FN)was detected by signal immunodiffusion Results:After intravenous injection of 1 3ml/kg fat tissue extract(FTE),the dogs in FES group immediately developed respiratory distress,the partial pressure of oxygen in artery(PaO 2)decreased and moist rales in both lung bases could be heard Pathologic studies demonstrated an increased number of inflammatory leukocytes,interstitial fat embolism and alveolar edema All the results indicated that we could establish a good animal model of FES at simulated high altitude with intravenous injection of 1 3ml/kg FTE What’s more,we found that(PaO 2/FIO 2)x (pressure of inspired air/air pressure at sea level) was less than 300,WBC and platelet count,PaO 2and FN decreased,but GPT,CD and SA increased Conclusion:Decrease of WBC and platelet count,FN content and PaO 2,increase of GPT,CD and SA and respiratory distress might be the premonitory index of FES at high altitude更多还原