【摘要】 用ELISA法对39只次发情雌性大熊猫尿中雌酮(E1G)和19只次产前大熊猫、6只次假孕大熊猫尿中孕酮(P4)的含量进行测定、统计分析,结果表明:(1)雌性成体大熊猫,发情期多在2—5月份,尿中E1G/Cr由基础值<12ng/mg开始上升,达峰值需8—15d,平均12d。E1G/Cr峰值>60ng/mg,峰值下降12h内进行首次本交、人工授精或两者交替配种,受孕率达100%;峰值下降12—24h内进行首次本交、人工授精或两者交替配种,受孕率达83 3%;峰值下降24h后进行首次配种,受孕率为0。E1G/Cr峰值<60ng/mg的发情雌性大熊猫,无论在峰前、峰值下降时或峰后进行首次配种,受孕率都为0。(2)大熊猫产仔季节一般多在7—9月份,妊娠期多为108—184d,平均150d。有妊娠行为表现时P4/Cr由基础值<12ng/mg开始上升,达峰值时平均需30d,P4/Cr峰值>60ng/mg。大熊猫产前9—18d出现P4/Cr峰值,然后急剧下降,达产仔平均需14d。(3)假孕大熊猫由配种到假孕结束所需时间与真孕无显著性差异。在整个假孕期间,P4/Cr波动性大,峰值不太明显,假孕行为表现持续时间平均为41d。更多还原

【Abstract】 Estrone βDGlucurojnide(E1G)level in 39 estrus female giant pandas urine samples,progesterone(P4) level in 19 antenatal female giant pandas urine samples and that in 6 pseudopregnant female giant pandas urine samples were studied and analyzed by ELISA.The results showed that:1)Female giant pandas estrus period is always from March to May.It lasts 815 days,during which the E1G/Cr level in urine rises from a base level(<12 ng/mg) to a peak(>60 ng/mg).The average length of estrus is 12 days and the highest E1G/Cr peak level is 799 ng/mg(±108 ng/mg).Following each peak comes a sudden drop in hormone levels,after which giant pandas mating three spearate times:once in 12 hours,once randomly between the 12 and 24 hour marks,and once 24 hours later.The impregnation rate is 100%,833% and 0%,consecutively.When the peak is lower than 50 ng/mg,pregnancy is not achieved.2)The birthing season of giant pandas is from July to September.Gestation ranges from 108 days to 184 days,and is on an average 150 days.Early on in gestation,the P4/Cr level is lower than 12 ng/mg.As the level rises,a panda begins to exhibit behaviors common to pregnancy.After about 30 days the hormone peaks(>60 ng/mg).9-18 days(average 14 days)before giving birth,the P4/Cr level drops sharply.3)The (pseudo) gestation period of pseudopregnant pandas is not significantly different than that of pregnant pandas.However,in pseudopregnancy,the P4/Cr levels fluctuate and lack a significant peak.On an average,psuedopregnant pandas exhibit pregnantlike behavior for 41 days.更多还原