【摘要】 以两组40个随机引物,筛选出16个重复性好的多态引物,对四川5个山羊品种、藏山羊两个生态类型、3个杂交群体和岩羊共计113只个体,进行随机扩增多态DNA(RAPD)分析。结果表明:16个引物扩增出67条带,其中54条带呈现多态,多态率为80 60%。山羊各群体共有条带为23条,山羊和岩羊共有条带为13条,岩羊有4条特异带。不同引物所扩增出的片段在各群体中分布频率不同。山羊群体间相似系数为0 8216 0 9362,其中,安哥拉山羊与建昌黑山羊杂交后代F2和F3之间相似系数最大,为0 9362。山羊各群体与岩羊相似系数为0 4996 0 5064。山羊群体间的遗传距离为0 0638 0 1784。山羊各群体与岩羊的遗传距离为0 4936 0 5403。高原型藏山羊与山谷型藏山羊间的遗传距离小(0 1005)、相似系数大(0 8995),序列为TTCCGAACCC引物扩增出的片段为750bp,仅在高原型藏山羊中出现,可作为区分这两个生态类型的遗传标记。更多还原

【Abstract】 DNA of 113 individuals from 5 different goat populations in Sichuan,2 ecologytype of Tibeten goat,3 hybrid groups and Pseudois nayaurs were amplified with 16 random primers selected from 40 primers.The results shown:67 bands were amplified,in which 54 bands were polymorphic.The rate of polymorphism was 8060 percent.23 bands were produced among all goat populations,13 bands were produced among goat populations and Pseudois nayaurs,and 4 bands existed only in Pseudois nayaurs.The frequencies of DNA fragments amplified by different primers were different among populations.The similarity coefficients between goat populations were from 08216 to 09362,highest between F2 and F3 of Angora goats×Jianchang black goats,09362.The similarity coefficients between Pseudois nayaurs and different goat populations were from 04996 to 05064.Those genetic distance were from 00638 to 01784 between goat populations,and from 04936 to 05403 between Pseudois nayaurs and different goat populations.The genetic distance (01005) was near,and the similarty coefficent (08995) was high between plateau Tibetan goats and MountainValley Tibetan goats.The DNA fragment of 750bp amplified with the primer sequence TTCCGAACCC exists only in Tibetan goats,which could be the marker primer that distinguish the two ecotypes.更多还原