【摘要】 采用快速扩增cDNA末端 (RACE)方法测定了我国超强毒株Harbin 1和经典弱毒株CJ80 1的非编码区。在两株病毒基因组A节段中 ,Harbin 1的 5′非编码区包括 10 0个核苷酸 ,CJ80 1包括 96个核苷酸 ;两个毒株B节段的 5′非编码区均含有 111个核苷酸。Harbin 1毒株A节段的 3′非编码区含 95个核苷酸 ,CJ80 1含 94个核苷酸。Harbin 1毒株B片段的 3′非编码区含 79个核苷酸 ,CJ80 1含 82个核苷酸。结构分析表明 :超强毒株Harbin 1与细胞适应株CJ80 1的 5′和 3′端非编码区在一级和二级结构上均存在差异。Harbin 1与欧洲超强毒株的关系最近 ,CJ80 1与欧洲细胞适应株同源性最高。IBDV非编码区结构特征的研究为进一步研究IBDV非编码区的功能 ,阐明其与病毒复制调控和毒力的关系奠定了基础。更多还原

【Abstract】 In order to study the structure and function of Non-coding regions of difference virulent strains of IBDV,the non-coding regions of vvIBDV Harbin-1 strain and cvIBDV CJ801 strain were determined by RACE method.cDNA fragments corresponding to difference strains were cloned and sequenced then compared the sequences between the difference strains of overseas.The results showed that,in segment A,the 5′NCR of Harbin-1 consists of 100 nt with regard to the start codon of VP5 gene and CJ801 consists of 96 nt.In segment B,the 5′-NCR spans 111nt to the start codon of VP1 gene in both stains.The 3′ NCR comprise 95 nt in segment A of Harbin-1 with regard to the stop codon of Vpx gene and 94 nt of CJ801.In segment B,the 3′NCR consists of 79 nt in Harbin-1 and 82 nt in CJ801 with regard to the stop codon of VP1 gene.Seen from primary and secondary structure,CJ801 is more similar to European cvIBDV strains and Harbin-1 belong to vvIBDV group.There are obvious differences between two strains,which may be relate with their replication and pathogenicity.This research will facilitate our studies on the function of 5′ and 3′ non-coding regions of IBDV with reversed genetics system.更多还原